INVESTIGADORES
FOSSATI Carlos alberto
artículos
Título:
The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow?s Milk Proteins
Autor/es:
CANDREVA ANGELA; SMALDINI P; CURCIARELLO R,; FOSSATI CA; DOCENA GH,; PETRUCCELLI S,
Revista:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2016 vol. 64 p. 1590 - 1599
ISSN:
0021-8561
Resumen:
Reactions to soy have been reportedin a proportion of patients with IgE-mediated cow?s milk allergy (CMA). In thiswork, we analyzed if Gly m Bd 28K/P28, one of the major soybean allergens, is across-reactive allergen with cow milkproteins (CMP). We showed that P28 was recognized byIgE sera from CMA patients and activated human peripheral basophils degranulation.Moreover, IgE sera of mice exclusively sensitized to CMP recognized P28.Splenocytes from sensitized animals secreted IL-5 and IL-13 when incubated withCMP or soy proteins, but only IL-13 when treated with P28. In addition, a skintest was strongly positive for CMP and weakly positive for P28. Remarkably,milk-sensitized mice showed hypersensitivity symptoms following sublingualchallenge with P28 or CMP. With the use of bioinformatics? tools seven putativecross-reactive epitopes were identified. In conclusion, using in vitro and invivo tests we demonstrated that P28 is a novel cross-reactive allergen with CMP.