Poly-(l-lactic acid) sheets seeded with diaphragmatic myoblasts overexpressing connexin 43 as a potential strategy for myocardial regeneration.

C.S. GIMENEZ; F.D. OLEA; P. LOCATELLI; M.R. BAUZA; A. ORLOWSKI; A. AIELLO; A. DE LORENZI; M.G. CASTILLO VELASQUEZ; R. DEWEY; F. MONTINI BALLARIN; G.A. ABRAHAM; L. CUNIBERTI; A. CROTTOGINI

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias 2017; 2017

SAIC-SAIB-SAI-SAA-SAB-SAB-SAFE-SAFIS-SAH-SAP

Introduction. The diaphragm contracts and relaxes 20 times perminute over a lifespan. Besides, it is a source of muscle stem cells(myoblasts) capable of dividing, differentiating and self-regeneratingpreserving their phenotype. This makes diaphragmatic myoblasts(DM) a potential candidate for cell-based cardiac regeneration.Methods. To test this hypothesis, we seeded DM modified to overexpressconnexin 43 (cx43) on poly-(l-lactic acid) (PLLA) sheets andsutured them on the infarcted zone of sheep undergoing coronaryligation (PLLA-DMcx43 group, n=6). DM were transduced with cx43to enhance inter-cell connection. Additional sheep received PLLAsheets with non-modified DM (PLLA-DM group, n=6), PLLA withoutDM (PLLA group, n=6) or no treatment (Placebo, n=6). LV ejectionfraction (Fey) (echocardiography) and infarct size (cardiac magneticresonance) were measured at 2 and 45 days post-AMI. Results.Infarct size decreased by ~25% in PLLA-MDcx43 [8.7±0.6 ml (day2) to 6.5±0.72 ml (day 45), P