PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI

M. SRINIVAS; J. TEL; G. SCHREIBELT; F. BONETTO; J. L. CRUZ; H. AMIRI; A. HEERSCHAP; C. G. FIGDOR; J. DE VRIES

NANOMEDICINE

FUTURE MEDICINE LTD

Lugar: Londres; Año: 2015 vol. 10 p. 2339 - 2348

1743-5889

AIMS: In vivo imaging using 19F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here we formulate two stable and clinically-applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.MATERIALS & METHODS: Plasmacytoid and myeloid DCs (pDCs and mDCs) are able to take up sufficient nanoparticles (200 nm) for imaging (1012 19F?s per cell), despite being relatively non-phagocytic.RESULTS: Clinically-relevant numbers of labeled DCs could be imaged in about 10 minutes, even on a clinical scanner.CONCLUSION: We demonstrate the use of PFC nanoparticles for simultaneous 19F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.