Lead effects on Brassica Napus photosynthetic organs

G. V. FERREYROA; M. G. LAGORIO; M. A. TRINELLI; R. S. LAVADO; F.V. MOLINA

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2017 vol. 140 p. 123 - 130

0147-6513

Version:1.0StartHTML:0000000250EndHTML:0000004087StartFragment:0000002676EndFragment:0000004047SourceURL:file:///G:FerZDropboxGiselePbSuelosPaperTesis3Chemosphere-EESManuscript_Ferreyroa_et_al_Lead%20effects_on_B_napus_Revised2.doc<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman";}@page Section1{size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.Section1{page:Section1;}-->In this study, effectsof lead on ultracellular structure and pigment contents of Brassica napus were examined. Pb(II) was added in soluble form tosoil prior to sowing. Pb contents were measured in plant organs at theontogenetic stages of flowering (FL) and physiological maturity (PM). Pigmentcontents were evaluated through reflectance measurements. Pb content in organswas found to decrease in the order; roots > stems > leaves. Lead contentin senescent leaves at FL stage was significantly higher than harvested leaves,strongly suggesting a detoxification mechanism. Leaves and stems harvested atthe PM stage showed damage at subcellular level, namely chloroplastdisorganization, cell wall damage and presence of osmiophilic bodies.Chlorophyll content increased in the presence of Pb at the FL stage, comparedwith control; at the PM stage, chlorophyll contents decreased with low Pbconcentration but showed no significant differences with control at high Pbsoil concentration. The results suggest an increase in antioxidants at low Pbconcentration and cell damage at higher lead concentration.