ARABIDOPSIS PROLINE DEHYDROGENASE PARTICIPATES IN THE ACTIVATION OF PATTERN TRIGGERED IMMUNITY

FABRO GEORGINA; ALVAREZ MARIA ELENA

Mendoza

Congreso; 48th Reunion Anual SAIB; 2012

Sociedad Argentina de Bioquimica y Biologia Molecular

Proline Dehydrogenase (ProDH) catalyzes the limiting step of proline degradation in the mitochondria. In yeast and animal cells, ProDH hyperactivity contributes to imbalances in the production of reactive oxygen species (ROS). In plants ProDH contributes to effector triggered immunity (ETI) in response to pathogens. In Arabidopsis ProDH is required for full development of the Hypersensitive Response (HR), a localized cell-suicide reaction against pathogens that is signaled and stimulated by ROS. Arabidopsis genome encodes two ProDH genes: and . The aim of this work was to investigate if these genes are also involved in activation of defences triggered by recognition of microbial patterns (Pattern Triggered Immunity, PTI). We used bacterial flagellin (flg22) to evaluate PTI activation.We found that flg22 induced an early and transient expression of but not . Single mutants for each gene showed reduced callose deposition at the cell wall in response to the peptide. The growth curves of a weakly virulent bacterial pathogen ( DC3000 DeltaCEL) indicated that as well as mutants are hyper-susceptible to this strain, which is unable to suppress PTI in wild type plants