INVESTIGADORES
ETCHEVERRIA Analia Ines
congresos y reuniones científicas
Título:
Prevalent STEC serotypes isolated from cattle, foods and environment in Argentina.
Autor/es:
PADOLA, NORA L.; ETCHEVERRÍA, ANALÍA I.; LUCCHESI, PAULA M.A.; KRÜGER ALEJANDRA; SANZ, MARCELO, E; FERNÁNDEZ, DANIEL; ALONSO, MÓNICA Z.; POLIFRONI, ROSANA; ARROYO, GUILLERMO H.; PARMA, ALBERTO E.
Lugar:
Amsterdam
Reunión:
Simposio; 8th International Symposium on Shiga Toxin (Verocytotoxin) producing Escherichia coli infections.; 2012
Institución organizadora:
Asociación Argentina de Micerobiología
Resumen:
STEC is the main aetiological agent of
haemolytic uraemic syndrome (HUS) and cattle are the main reservoir of STEC
that is transmitted to humans through contaminated foods, water, direct contact
with animals and the environment. Although the serotype more frequently implicated in HUS is O157:H7,
there are several other serotypes associated with human infections: O26:H11/H-,
O91:H21/H-, O103:H2, O111:H-, O113:H21, O118:H16, O121:H19, O128:H2/H-,
O130:H11, O141:H19, O145:H28/H-, O146:H21, O163:H19, O172:H-, and O178:H19.
Argentina has the highest incidence of HUS
(17/100,000 children under five years old), and several serotypes have been
isolated from human cases in this country: O157:H7, O145:H-, O178:H19, O91:H21
and serogroups as O26, O174, and O111.
The aim of this work was to determine the
serotypes shared among cattle, foods and environment and assess which are the
most prevalent in a collection of STEC isolated in Argentina.
The isolates came from 4824 samples from
cattle, foods (hamburger and minced meat) and environment of farms. From those,
545 were characterized by multiplex PCR to detect stx1, stx2, eae, ehxA
and saa and then were serotyped.
The prevalent serotypes were O8:H19; O26:H11;
O91:H21; O113:H21; O117:H7; O130:H11; O145:H-; O157:H7; O171:H2 and O178:H19,
corresponding to 61 % of typable strains. There were serotypes shared between cattle and
foods, between cattle and the environment and among cattle, foods and
environment. Ninety eight seroytpes (18%) were non-typable (Table 1).
Serotype
Percentage of strains (%)
Virulence
profiles
(n=447)
Cattle
Foods
Environment
O178:H19
13
95
5
0
stx2
stx2
saa ehxA
stx1
stx2 saa ehxA
O130:H11
9
93
7
0
stx1
stx2 saa ehxA
stx1
saa ehxA
O113:H21
8
86
14
0
stx2
saa ehxA
stx1
stx2 saa ehxA
stx1
eae ehxA
stx2
O26:H11
5
91
0
9
stx1
eae ehxA
stx2
eae ehxA
O91:H21
5
95
5
0
stx2
saa ehxA
O171:H2
5
86
14
0
stx2
O117:H7
3
50
50
0
stx2
O145:H-
3
93
0
7
stx1
eae ehxA
stx2
eae ehxA
stx1
eae
stx2
eae
O157:H7
3
93
7
0
stx2
eae ehxA
O8:H19
2
45
45
10
stx1
stx2 ehxA
stx1
ehxA
stx2
The most prevalent serotypes from cattle, foods
and environment in Argentina
have been also isolated from human cases in several countries including Argentina, and
their virulence profiles reflect the pathogenic potential of the strains.