MINTHOSTACHYS VERTICILLATA ESSENTIAL OIL ALONE OR MICROENCAPSULATED ACTIVATES TH1 CELLS AND INDUCES THE PRODUCTION OF OPSONIZING ANTIBODIES

CAMPRA N.; MOLINA M.V.; ARSAUTE S.; RAVIOLO J.M.; CAMACHO N.M.; PAREDES A.J.; MORAN M.C.; MALETTO B.; PALMA S.; ESTEIN S.M.; CARIDDI L.N.

MAR DEL PLATA

Otro; Reunión Anual de Biociencia 2020, SAIC. SAFE. SAB. SAP. AACYTAL. NANOMED-ar. HCS.; 2020

SAIC. SAFE. SAI.

In a previous study, we demonstrated the adjuvant capacity of the Minthostachys verticillata essential oil (EO) (2.5 mg/ml) or microencapsulated essential oil (EOMC) (5 mg/ml) on the humoral and cellular immune response against Enterococcus faecium in Balb/c mice. The aim of this study was to evaluate the functionality of the specific antibodies produced and to quantify the IFN-γ and IL-4 cytokines. Balb/c mice (n=4 per group) were inoculated by subcutaneous injection (days 0, 14 and 28) as follows: Group 1: 100 μl saline solution; Group 2: 100 μl bacterin+EO (2.5 mg/ml); Group 3: 100 μl bacterin+ microcapsule wall material (2.5 mg/ml); Group 4: 100 μl bacterin+EOMC (5 mg/ml). In all groups, bacterin was of E. faecium. Seven days after the last booster, blood was obtained and serum was separated. Mice were sacrificed by cervical dislocation and spleens were removed to obtain splenic lymphocytes for cell proliferation assay. To evaluate the functionality of the antibodies, the Opsonophagocytic Killing Assay was carried out using differentdilutions of the sera (1/100, 1/200, 1/500, 1/1000, 1/2000), E. faecium (1x107 CFU/ml), bovine PMNs (1x107cells/ml) suspensions and a source of bovine complement. Sera antibodies of mice fromGroup 4 mediated the opsonization of E. faecium and enhance the phagocytosis by PMNs up to the 1/500 dilution compared to Group 2, which was up to the 1/200 dilution (p