ESPELT Maria Victoria
Natural flavonoidNatural flavonoids inhibit the plasma membrane Ca2+-ATPase
ONTIVEROS M; RINALDI DE; MARDER M; ESPELT MV; MANGIALAVORI I; VIGIL M; ROSSI, JP; FERREIRA-GOMEZ
PERGAMON-ELSEVIER SCIENCE LTD
Lugar: Amsterdam; Año: 2019 vol. 166
Recently, there has been increasing interest in the research on flavonoids from plant sources because of their beneficial properties for health. Different studies have shown that flavonoids change the intracellular Ca2+ homeostasis linked to alterations in the function of mitochondria, Ca2+ channels and Ca2+ pumps. In this sense, the Plasma Membrane Ca2+-ATPase (PMCA) could be involved as it transports Ca2+ actively to the extracellular medium coupled to the ATP hydrolysis maintaining the ion cellular homeostasis. The present study search for investigating the effect of several natural flavonoids on PMCA both in isolated protein systems and in living cells, and to establish the relationship between the flavonoid structure and its inhibitory activityfor PMCA.Our results indicated that natural flavonoids inhibited purified and membranous PMCA with different effectiveness:quercetin and gossypin were the most potent and their inhibition mechanisms seem to be different since quercetin does not prevent the ATP binding whereas gossypin does. Moreover, the PMCA activity was inhibited in in human embryonic kidney cells, whichtransiently overexpress PMCA, suggesting that the effects observed on isolated systems could occur in a complex structure like a living cell. In conclusion, this work reveals a novel molecular mechanism through which flavonoids inhibit PMCA andconsequently Ca2+ homeostasis and signaling in the cell would be affected.