Reactive intermediates and arsenite cytotoxicity on CHO-K1 cells

SORIA EA; EYNARD AR; BONGIOVANNI GA

Huerta Grande (Argentina)

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

Oxidative stress has been increasingly recognized as a possible mechanism in the arsenic toxicity and carcinogenicity. Previous results showed a protective effect of flavonoids against in vitro arsenical cytotoxicity on CHO-K1 cells treated with 200 µM NaAsO2 (As) and/or flavonoid (5 µM silymarin, S; 50 µM quercetin, Q). The objective was to evaluate stress markers induction and cell death by arsenite. It was studied: cellular viability (CV), hydroperoxides (HP, aqueous and lipidic), L-citrulline and JNK (c-Jun N-terminal kinase) activation. Statistical analyses were performed by the Info Stat 2007e.1 software (p