Retinoic acid and fatty acids: Effects on human breast cancer cells

QUIROGA PL; EYNARD AR; VALENTICH MA; SORIA EA

Huerta Grande (Argentina)

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

Introduction: The unsaturated fatty acids (UFA) and the all-trans retinoic acid (RA) could play a modulating role on the carcinogenic process, but their combined use has not been completely studied. Objective: To modulate tumor parameters in ZR-75-1 cells by RA and/or UFA (families: n-3, n-6 y n-9). Methodology: The cells were treated with RA 1 µM and UFA 50 µM: eicosapentaenoic (EPA, n-3), γ-linolenic (GLA, n-6), oleic (OA, n-9) or eicosatrienoic (ETA, n-9). It was assessed cellular proliferation (CP: MTT assay), eicosanoid production (HPLC), lipid profile (GC) and E-cadherin expression (Immunocytochemistry). Statistics: ANOVA + LSD Fisher test (p