The Global Durum Panel (GDP) and the Tetraploid wheat Global Collection (TGC): two germplasm resources to leverage native variability of the A and B wheat QTLome for pre-breeding activities

MACAFERRI M; MAZZUCOTELLI E; SCIARA G; MASTRANGELO A; DESIDERIO F; XU S; HAYDEN M; TRICKER P; OZKAN H; ECHENIQUE V; STEFFENSON B; KNOX R; NIANE A; UDUPA S; LONGIN F; MARONE D; PETRUZZINO G; CORNETI S; ORMANBEKOVA D; POZNIAK C; RONCALLO P; MATHER D; ABLE J; AMRI A; BRAUN H; AMMAR K; BAUM M; CATTIVELLI L; BASSI F; TUBEROSA R

Zhengzhou

Congreso; Triticeae genomics and applications in wheat molecular breeding; 2021

chinese academy of agricultural science

Two germplasm collections representing the largest native, molecularly profiled (Illumina 90K SNP array) diversity of tetraploid wheat were assembled for complementary purposes.A breeding-dedicated collection (1,033 accessions), named Global Durum Panel (GDP), assembled through a ?bottom-up approach?, represents the genetic diversity of durum breeding programs worldwide. The GDP includes mostly modern T. durum accessions and landraces, with a minor component of wild emmer and domesticated tetraploid wheats, all SSD-purified and increased at ICARDA. GDP is suitable for pre-breeding and breeding activities while providing opportunities for mapping studies (Mazzucotelli et al., 2021, Frontiers). An evolutionary-dedicated collection (1,856 SSD-purified accessions), named Tetraploid wheat Global Collection (TGC), is particularly suited for surveying native variation of tetraploid wheat, gene discovery, allele mining and exploratory pre-breeding activities. The TGC, assembled through a ?top-down approach?, includes wild emmer, domesticated emmer, T. turgidum landraces, T. turanicum, polonicum, carthlicum and other germplasm based on GenBank passport information (Maccaferri et al., 2019 Nature Genetics). Details on both collections are reported in GrainGenes. The TGC is being characterized for resistance to yellow rust (YR), leaf rust (LR), soil-borne cereal mosaic virus (SBCMV) and other traits of agronomic interest such as root system architecture. Major QTLs for resistance to YR, LR and SBCMV have been mapped down to a resolution of 1.5 Mb each. Three major QTLs for root growth angle have been consistently identified through emmer and durum GWAS panels. The availability of the cv. ?Svevo? durum wheat assembly greatly facilitates candidate gene discovery and the design of diagnostic haplotypic KASP® assays for breeding purposes. The GDP and TGC provide a valuable opportunity to bridge native variability between tetraploid and hexaploid wheat.