DINOLFO Maria Ines
ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England
DINOLFO M.I; STENGLEIN S.A; MORENO M.V; NICHOLSON P.; JENNINGS P.; SALERNO G.L
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Lugar: Berlin; Año: 2010 vol. 127 p. 483 - 491
Fusarium poae is one of the Fusarium species isolated from grains of cereals afflicted by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n=62) and England (n=35) were analyzed by Inter-Simple Sequence Repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. F. poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe a combination of subclusters consisting of isolates only from Argentina or England. Furthermore, a single seed sample was found to be able to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability resulting from differences within, rather than between Argentinian and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.