Interaction of the chlorogenic acid (CGA) with model lipid membranes and its influence in antiradicalary activity

CEJAS, J. P.; DISALVO E.A.; FRIAS, M. A

San Luis

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; 2019

Chlorogenic acid (CGA) presents strong antioxidant and antibacterial properties reducing the risk of diabetes, fungal infections and cancer among others benefits, and also protects the liver and other organs. CGA is an important natural antioxidant composed by an ester of caffeic and quinic acid, that belongs to the group of polyphenols. A high antioxidant activity with respect to the red blood cell membranes has also been reported [2], but its mechanism of action is not yet well defined. In this work, the interaction of CGA with model lipid membranes DMPC, DPPC and 14:0 Diether PC monolayers) was studied through changes in surface pressure ()and the analysis of the kinetics of interaction. In addition, the absorbance reduction of the radical cation ABTS?+ after the addition of CGA in absence and presence of unilamellar vesicles (LUVs) was followed by spectrophotometry. These results allow us to discuss a possible mechanism of interaction of CGA with lipid membranes depending on the hydration state of the lipid interface and according to this infer about its influence on the ARA.