Response of water behavior at the interphase of phospholipid matrixes assessed by FTIR

A.S. ROSA; E. A. DISALVO; M. A. FRÍAS

Buenos Aires

Workshop; Structure and Dynamics of Glassy, Supercooled and Nanoconfined Fluids; 2019

Lipid membranes are one of the most important biological matrixes in which biochemical processes take place. This particular lipidarrangement is driven by different water disposition interacting with it, which is related to different water states with different energylevels at this interphase.In our work we report changes in water content and distinctive water states by FTIR when this self assembled matrix changes itsphysicochemical properties. The OH stretching band in the liquid water IR spectrum is the principal region used to understand its molecularorganization (4000-3000cm-1). The strength of the hydrogen-bonding depends on the cooperative/anti-cooperative nature of thesurrounding hydrogen bonds, with strongest hydrogen bonds giving the lowest vibrational frequencies. Thus, we can use water as mirror ofthe membrane state in this kind of biological systems.Different phospholipids associate water at particular modes according to their structure; this may produce modulation of packing andhydration suitable for the incorporation of aminoacid of peptides and enzymes.