Studies on the structure of FQs species present at different pH´s and their interactions with lysozyme protein

HUGO ALEJANDRO PEREZ; LEDESMA, ANA; FRIAS, M. DE LOS ANGELES

Buenos Aires

Congreso; XLVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE BIOFÍSICA (SAB).; 2017

LA SOCIEDAD ARGENTINA DE BIOFÍSICA

Fluoroquinolones (FQs) are synthetic antimicrobial agents with a broad spectrum of antibiotic activity against Gram-positive and Gram-negative bacteria. They play an important role in the treatment and prevention of disease in both humans and animals. On the other hand, patients often take antibiotics with foods or dairy products such as milk to help swallow easier and to decrease their gastrointestinal side effects. Therefore FQs may be in contact with proteins present in milk as lysozyme (Lyz). It is known, that food?drug interactions may occur by many mechanisms, and they can result in changes both in the rate and the extent of absorption. The pH of the gastric milieu may also be an important determinant of the magnitude of the interaction.In this context the purpose of this work is to study the structure of Ciprofloxacin (Cpx) and Levofloxacin (Lev) species present at basic, acid and neutral pH´s and their interactions with Lyz structure by Fourier transform infrared spectroscopy (FTIR) and Theoretical Calculations.