Study of Antimicrobial Action of Aguaribay Essential Oil on Staphylococcus aureus

ANDREA C. CUTRÓ; CASTELLI, MARÍA VICTORIA; ROSALES, MANUEL; LÓPEZ, SILVIA NOELÍ; HOLLMANN, AXEL; RODRIGUEZ, SERGIO

La Plata

Congreso; XLVII Reunión anual de la Sociedad Argentina de Biofísica; 2018

Sociedad Argentina de Biofísica

Microorganisms have developed resistance to many antibiotics and as a result,immense clinical problems in the treatment of infectious disease have beenemerged. A key strategy to control antibiotic resistance is to stimulate thedevelopment of novel antibacterials to continue treating resistant infections. Inparticular, the antimicrobial activities of extracts and plant essential oil haveformed the basis of many alternative medicines and natural therapies. Essentialoils of plant origin are among the best known substances to have attractedattention in recent years for you several uses like as potential pest control agents,food preserving, antibacterial, antioxidant and antifungal.The genus Schinus(Anacardiaceae) is a native species from the north-west region of Argentina.It hasbeen reported many traditional uses for S. areira L. like as a purgative, diuretic,parasiticide, insecticide, vulnerary, and topical disinfectant, and for the treatmentof rheumatism, stomach upsets, menstrual disorders, bronchitis and conjunctivitis,treatment of colds, as an expectorant, among others. In this work, the essential oil(EO) chemical composition of S. areira from Northwest of Argentina has beendetermined and its antimicrobial potential. Bioautography assay has been shown,that S. areira EO has antibacterial activity on S. aureus, producing inhibitory effectat 25, 50 and 100 µg. The minimum inhibitory concentration was 62.5 µg.mL-1,showing that at 256 µg.mL-1 a bacteriolytic action. Further studies on theinteraction of S. aureus EO with artificial membranes, such as phosphatidylcholineand phosphatidylglycerol liposomes (PC:PG; 5:1 MLVs), indicates that itsinteraction, produce change in electrical properties of the membrane, producingan increase in Zeta potential of 6 mV. This could indicate that interaction withmembrane could be one of target for its antimicrobial mechanism of action.