The interaction of ph sensitive liposomes with lung surfactant in monolayer biomimetic systems?

MARIA JULIA ALTUBE; ANDREA C. CUTRÓ; ANIBAL E. DISALVO; ROMERO, EDER L.

Mar del Plata

Congreso; 2016 LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2016

Sociedad Argentina de Investigación Clínica (SAIC).

The archaeosomes (ARC) are lipid vesicles made of total polar archaeolipids (TPA) extractedfrom hyperhalopile archaebacteria (1). Previous studies have shown that ARC are moreextensively captured by alveolar macrophages and lung epithelial cells in vitro thanconventional liposomes (2). This makes ARC excellent candidates for delivering drugs to thelungs. Although, in an in vivo context inhaled ARC must first interact with the pulmonarysurfactant (PS) lining layer that covers the internal surface of the alveolus and provides thelow surface tension at the air-liquid interface that is necessary to prevent collapse duringexpiration. Interactions with the PS film determine the subsequent retention or translocation ofthe inhaled ARC and hence their potential activity on target cells (3). In order to evaluate theeffect of the interaction of ARC, the changes in surface pressure (π) and compressibilityproduced in lung surfactant monolayers by ARC addition were studied. The results show thatARC increases the π, indicating its incorporation which is dependent of the membranepacking because the Δπ observed at high surface pressure (40 mN/m) are negligible incomparison to those obtained at low surface pressure (10 mN/m), (Δπ=4.5 mN/m). Besides,the ARC concentration is crucial to produce changes in pressure monolayers, at lowconcentration (0.3 µg/ mL) no significant changes were observed. Moreover, the ARCincorporation rates into the membrane was substantially affected if the addition done onto themonolayer in comparison to that in the subphase being the first much higher than the second.(1) Gonzalez, R.O., Higa,L. H., Cutrullis, R.A., Bilen, M., Morelli, I., Roncaglia, D.I., & Romero, E.L. (2009). BMCbiotechnology, 9(1), 71.(2) Altube, M.J., Selzer, S.M., de Farias, M.A., Portugal, R.V., Morilla, M.J., Romero, E.L. (2016). Nanomedicine,11(16):2103-17.(3) Gehr, P., & Tsuda, A. (2014). Nanoparticles in the Lung: Environmental Exposure and Drug Delivery, 21.