Molecular tools for biodiversity and phylogenetic studies in mycorrhizas: The use of primers to detect arbuscular mycorrhizal fungi

COVACEVICH, F

Perspectives in Mycorrhizal Research

Bioscience Publications (Eds. Thangadurai E, Hijri M, Busso CA)

Lugar: India; Año: 2010; p. 187 - 202

Some limitations (morphological characters, in some cases absence of spores, obligate symbiotic relationship) often lead to underestimate arbuscular mycorrhizal fungal (AMF) diversity, population number and species richness in the field. Molecular techniques based on DNA analysis seem to offer a wide range of advantages. Furthermore, the combination of molecular biology methods may be the most promising way to monitor community structure and biodiversity of AMF in the field. Much information about rDNA sequences with the species specific name has been accumulated in a gene bank NCBI(http://www.ncbi.nlm.nih.gov). It was also posible to confirm the biological species with their specific primers. However, there is no information about the primers commonly used for phylogenetic and biodiversity studies of arbuscular mycorrhizal relationships, and in some cases information seems to be repeated. This chapter collects information from spores to mycorrhizal roots about tue most usefull primers for phylogenetic and biodiversity studies of AMF