INVESTIGADORES
BUCALA Veronica
congresos y reuniones científicas
Título:
Dynamics of a marine-microbial community during biodegradation of bilge waste hydrocarbons
Autor/es:
NIEVAS, MARINA; FERRERO, M; OLIVERA, NELDA; DIONISI, H; COMMENDATORE, MARTA; ESTEVES, JOSÉ LUIS; BUCALÁ, VERÓNICA
Lugar:
Puerto Madryn
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2010
Institución organizadora:
SAIB
Resumen:
The aim of this study was to identify the dominant taxons of a
hydrocarbon-degrading microbial community enriched from bilge
waste oily phase, and to assess its population dynamics during bilge
waste biodegradation. Samples were retrieved from an aerated
batch bioreactor with 2.1 g/L total hydrocarbons in seawater
medium during a 14-day biodegradation experiment. Total DNA
was analyzed by PCR-DGGE, and the relative intensity of each
dominant band calculated. Members of the genus MarinobacterMarinobacter
were dominant in the enrichment, and Pseudomonas, ShewanellaPseudomonas, Shewanella
and Halomonas were also detected. During the biodegradation
experiment, the exponential-growth phase agreed with n-alkane
depletion and an increase in the prevalence of Pseudomonas and
experiment, the exponential-growth phase agreed with n-alkane
depletion and an increase in the prevalence of Pseudomonas and
Halomonas were also detected. During the biodegradation
experiment, the exponential-growth phase agreed with n-alkane
depletion and an increase in the prevalence of Pseudomonas andPseudomonas and
Shewanella. Only after emulsification, biodegradation of other
more recalcitrant hydrocarbons found in an unresolved complex
mixture (UCM) occurred, associated with a predominance of
more recalcitrant hydrocarbons found in an unresolved complex
mixture (UCM) occurred, associated with a predominance of
. Only after emulsification, biodegradation of other
more recalcitrant hydrocarbons found in an unresolved complex
mixture (UCM) occurred, associated with a predominance of
Marinobacter and Shewanella. Cluster analysis from DGGE
fingerprints showed shifts in the microbial community structure
which matches with the pattern of sequential hydrocarbon
biodegradation found (n-alkanes-UCM). Shewanella, a genus
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.
fingerprints showed shifts in the microbial community structure
which matches with the pattern of sequential hydrocarbon
biodegradation found (n-alkanes-UCM). Shewanella, a genus
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.
and Shewanella. Cluster analysis from DGGE
fingerprints showed shifts in the microbial community structure
which matches with the pattern of sequential hydrocarbon
biodegradation found (n-alkanes-UCM). Shewanella, a genus
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.
Shewanella, a genus
which can use diverse electron acceptors such as metals and
colonizes emulsified oil from spills, showed high prevalence during
both n-alkane and UCM biodegradation of bilge wastes, suggesting
a promising potential for bioremediation.