INVESTIGADORES
CORRADI gerardo Raul
artículos
Título:
Mitogenic Effects of Phosphatidylcholine Nanoparticles on MCF-7 Breast Cancer Cells
Autor/es:
YAMILA B. GÁNDOLA; SEBASTIÁN E. PÉREZ; PABLO E. IRENE; ANA I. SOTELO; JOHANNA G. MIQUET; GERARDO R. CORRADI; ADRIANA M CARLUCCI; LORENA GONZALEZ
Revista:
BioMed Research International
Editorial:
Hindawi Publishing Corporation
Referencias:
Año: 2014 vol. 2014 p. 1 - 13
ISSN:
2314-6133
Resumen:
Lecithins, mainly composed of the phospholipids phosphatidylcholines (PC), have many different uses in the pharmaceutical and
clinical field. PC are involved in structural and biological functions as membrane trafficking processes and cellular signaling.
Considering the increasing applications of lecithin-based nanosystems for the delivery of therapeutic agents, the aim of the present
work was to determine the effects of phosphatidylcholine nanoparticles over breast cancer cellular proliferation and signaling. PC
dispersions at 0.01 and 0.1% (w/v) prepared in buffer pH 7.0 and 5.0 were studied in theMCF-7 breast cancer cell line. Neutral 0.1%
PC-derived nanoparticles induced the activation of the MEK-ERK1/2 pathway, increased cell viability and induced a 1.2 fold raise
in proliferation. These biological effects correlated with the increase of epidermal growth factor receptor (EGFR) content and its
altered cellular localization. Results suggest that nanoparticles derived from PC dispersion prepared in buffer pH 7.0 may induce
physicochemical changes in the plasma membrane of cancer cells which may affect EGFR cellular localization and/or activity,
increasing activation of theMEK-ERK1/2 pathway and inducing proliferation. Results from the present study suggest that possible
biological effects of delivery systems based on lecithin nanoparticles should be taken into account in pharmaceutical formulation
design.