PERSONAL DE APOYO
CORNEJO paula
artículos
Título:
Molecular bases of the postzygotic barriers in interspecific crosses between the wild potatospecies Solanum acaule and Solanum commersonii
Autor/es:
P. CORNEJO; E. L. CAMADRO; R. W. MASUELLI
Revista:
GENOME
Editorial:
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
Referencias:
Lugar: Otawa; Año: 2012 vol. 55 p. 605 - 614
ISSN:
0831-2796
Resumen:
To investigate the molecular bases of postzygotic hybridization barriers in tuber-bearing Solanums, the wild speciesSolanums, the wild species
Solanum commersonii Dunal ex Poir. (cmm, 2n = 2x = 24, 1EBN) and Solanum acaule Bitter (acl, 2n = 4x = 48,
2EBN) were crossed in intra- and interspecific genotypic combinations, and the transcriptome of immature seeds was analyzed
by using the cDNA-AFLP technique. From a total of 423 analyzed cDNA fragments, 107 (25.3%) were differentially
regulated in the compatible (acl × acl and cmm × cmm) versus incompatible (acl × cmm) crosses. DNA sequence data
were obtained from 21 fragments and RT?PCR analyses were carried out with five fragments to validate the cDNA-AFLP
differential pattern. Sequence analysis suggested a possible role for the differentially expressed sequences in cytokinesis, cell
cycle, secondary and hormonal metabolism, biodegradation, and transport. In situ hybridization experiments with fragments
encoding an ubiquitin-fold modifier 1 precursor and a possible vesicle transport protein revealed expression of these genes
in the embryo and endosperm. The results suggest that the collapse of the embryo and endosperm in incompatible crosses
may be related to alterations in cell cycle and cytokinesis.Dunal ex Poir. (cmm, 2n = 2x = 24, 1EBN) and Solanum acaule Bitter (acl, 2n = 4x = 48,
2EBN) were crossed in intra- and interspecific genotypic combinations, and the transcriptome of immature seeds was analyzed
by using the cDNA-AFLP technique. From a total of 423 analyzed cDNA fragments, 107 (25.3%) were differentially
regulated in the compatible (acl × acl and cmm × cmm) versus incompatible (acl × cmm) crosses. DNA sequence data
were obtained from 21 fragments and RT?PCR analyses were carried out with five fragments to validate the cDNA-AFLP
differential pattern. Sequence analysis suggested a possible role for the differentially expressed sequences in cytokinesis, cell
cycle, secondary and hormonal metabolism, biodegradation, and transport. In situ hybridization experiments with fragments
encoding an ubiquitin-fold modifier 1 precursor and a possible vesicle transport protein revealed expression of these genes
in the embryo and endosperm. The results suggest that the collapse of the embryo and endosperm in incompatible crosses
may be related to alterations in cell cycle and cytokinesis.?PCR analyses were carried out with five fragments to validate the cDNA-AFLP
differential pattern. Sequence analysis suggested a possible role for the differentially expressed sequences in cytokinesis, cell
cycle, secondary and hormonal metabolism, biodegradation, and transport. In situ hybridization experiments with fragments
encoding an ubiquitin-fold modifier 1 precursor and a possible vesicle transport protein revealed expression of these genes
in the embryo and endosperm. The results suggest that the collapse of the embryo and endosperm in incompatible crosses
may be related to alterations in cell cycle and cytokinesis.