DETERMINATION AND IN VITRO BIOACCESSIBILITY ASSESMENT OF NUTRIENTS IN FOOD USED TO RAISE MONOGASTRICS ANIMAL.

CORA JOFRE, F.; LARREGUI, D. N.; SOSA, A. P.; ROSSINI, A.; NEHER, B.D; SAVIO M.

Caldas Novas

Congreso; 19° ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA 7° CONGRESSO IBEROAMERICANO DE QUÍMICA ANALÍTICA; 2018

Animal feed mineral content is often high; however, their bioavailability in monogastric organism is poor due to the presence of antinutritional factors. Thus, certain grains are subjected to processing treatments to impart special characteristics and improve organoleptic properties. All these actions result in their nutritional quality alteration, which could bring antinutrients or phytochemicals reduction, digestibility improvement or nutrients availability.Bioavailability denotes the substance fraction that reaches the systemic circulation from the gastrointestinal (GI) tract (bioavailable fraction), and is available to promote its action in an exposed organism. A primary step in bioavailability assessment is the bioaccessibility study, which point out the analyte maximum fraction in food that is theoretically released from its matrix in the GI tract (bioaccessible fraction), and thus becomes available for intestinal absorption (i.e. enters the blood stream). Bioaccessibility could be evaluated through in vitro methods providing an effective approximation to in vivo situations and offering the advantages of good reproducibility, simplicity,rapidity, as it is possible to control conditions better than with in vivo tests. Due to the importance of knowing the real nutritional value of animal feed, the main goal of this work was to evaluate the total concentrations and bioaccessibility of nutrients in balance meal used to raise pigs. Eleven analytes (Al, Ba, Ca, Cu, Fe, K, P, Mg, Mn, Sr and Zn) were determinedusing microwave induced plasma atomic emission spectrometry (MPAES). A physiologically based extraction test was performed, simulating digestion in the gastric and gastrointestinal phase. A modified procedure similar to that used in several other research studies was adopted, simulating gastric and gastrointestinal digestions. The stomach and intestinal bioaccessible concentrations were calculated by dividing the analyte concentrations measured in the in vitro gastric phase or in the in vitro intestinal phase solutions by the total concentrations. The highest total content, in mg.Kg−1, were observed for Ca (3900?6423) and P (3136? 3859), whereas Ba (6?17), Sr (13−37) and Cu (9?104) presented the lowest concentrations. The highest bioaccessible fractions in gastric and gastrointestinal phases were observed for Cu, Mg and Mn. This study provides information for a better bioaccesibility understanding in monogastrics animal feeding and a more realistic estimation of analytes exposure, thus it allows better comprehend the nutritional alterations that occur due to the food preprocessing treatments.