SUB-LETHAL EFFECTS OF A GLYPHOSATE-BASED HERBICIDE ON Daphnia spinulata Birabén, 1917 AT DIFFERENT SALINITIES

LARES, B; VIGNATTI, A.; ECHANIZ, S.; CABRERA, G.; GIACOMINO, V.; CORA JOFRE, F.; GUTIERREZ, M

San Luis

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Cereal and oilseed production is one of the most important economic activities in the Argentine Pampas region. It and involves the use of large amounts of glyphosate (N- (phosphonomethyl) glycine) for crop protection. Part of the residues of this herbicide can reach nearby water bodies and put non-target aquatic organisms at risk, including Daphnia spinulata (suborder Cladocera), an endemic species of shallow, subsaline lakes in the region. The present study’s objective was to evaluate the effects of an herbicide (Panzer Gold®) on the life cycle parameters of this species. Two treatments were carried out, with 0.11 mg/L (T1) and 3.75 mg/L (T2) of herbicide in two concentrations of salts, 1 g/L (S1) and 2 g/L (S2), plus their respective controls. Neonates less than 24 hours old were placed individually in containers with 25 ml of solution and kept until death under a light/darkness regime of 16:8 and a temperature of 22 ±1 °C. Molts and offspring were measured and removed every 48 hours, the medium was changed, and the specimens were fed with Chlorella vulgaris.Significant differences (p