OXIDATIVE STRESS BY GLYPHOSATE (COMMERCIAL FORMULATION PANZER GOLD) IN Boeckella poopoensis MARSH, 1906 (CRUSTACEA, COPEPODA)

CABRERA, G.; VIGNATTI, A.; ECHANIZ, S.; NEGRO, L.; GUTIERREZ, M; CORA JOFRE, F.

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

Glyphosate (N-(phosphonomethyl) glycine) is the most widely used herbicide worldwide for control weed. In South America, particularly in the pampean region, where an important agricultural production is developed, large quantities are used each year. Although its toxicological risk is disputed, various studies have shown its harmful effects on aquatic organisms. Given that Boeckella poopoensis is a frequent and representative calanoid of the saline lakes of the Neotropical region, the aim of this work was to evaluate the effect of this herbicide on the antioxidant mechanisms of the species. An experiment was developed to evaluate the enzymatic activity of catalase (CAT) and glutathione S transferase (GST) and the levels of hydrogen peroxide (H2O2). For 15 days, copepodites IV and V males and females separately, were exposed to four concentrations of glyphosate (commercial formulation Panzer Gold): 0 (control); 0.58; 1.16 and 2.32mg/L.For each treatment, 4 replications were carried out with 15 individuals each, transparent bottles of 100 ml were used. Every 48 h, the medium was renewed (mesocosm water, previously sterilized, where a stable B. poopoensis population is maintained) and Dunaliella salina was fed. The photoperiod was 8/16 hours (darkness/light) and the temperature was 22 (±1) °C. After exposure, a significant difference (p