INVESTIGADORES
CONTIN Maria Ana
artículos
Título:
Chicken Embryo Retinal Ganglion Cells, A Tool For Neuronal Research
Autor/es:
PANZETTA PEDRO; BROCCO MARCELA A; CONTIN MARIA ANA; CARPENTIERI AGATA RITA; GUIDO MARIO E
Revista:
Current Trends In Neurology
Editorial:
Current Trends In Neurology
Referencias:
Año: 2006 vol. 2 p. 47 - 57
ISSN:
0972-8252
Resumen:
Retinal ganglion cells (RGCs) are the only
projecting neurons in retina. Taking advantage of
having these cells purified, we have performed
developmental, biochemical and circadian
studies. Anti-chicken Thy-1 sera were raised to
accomplish panning purification of RGCs. From
7-days-old embryo (E7) retina, we obtained two
different populations of RGCs. By gentle washing
and successive panning, we obtained a
population of antibody loosely bound cells
(LBCs), mostly immature. And after exhaustive
washing we harvested a small population of
tightly bound cells (TBCs), characterized as
mature RGCs. Latter cells cultured at low density
in a tailored growth factor lacking medium,
survived and regrew their processes. Evaluation
of process regrowth was determined by
neurofilament cell-ELISA. Substrata of several
extracellular matrix proteins modulated the cell
development. In cultures performed on
thrombospondin, a disintegrin (flavoridin)
produced a dose-response inhibition, suggesting
an integrin-thrombospondin receptor(s)
interaction(s) on process regrowth. Neurotrophic
factors (NTFs) and depolarization improved
regrowth on LBC cultures, while they did not
affect survival and regrowth on TBC cultures.
Moreover, addition of multiple NTFs caused cell
death in latter cultures. Consequently, chicken
RGC system would approach insight on
phenomena elicited by NTFs in CNS neurons,
which evoke survival and death pathways.
Cultures of RGCs from E8 retina, in which the
photoreceptors have not still developed
displayed a circadian fluctuation in phospholipid
labeling and synthesized melatonin?like indole.
They showed circadian rhythms in the
expression of mRNA of arylalkylamine Nacetyltransferase,
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis. Therefore, RGCs would constitute
primary retinal circadian oscillators.
a key enzyme in melatonin
synthesis.