" Prenatal xenoestrogen exposure affects amh, sox9 and sf-1 mRNA expression in Caiman latirostris?

COCITO, LAURA L.; DURANDO MILENA; RODRIGUEZ HORACIO; VARAYOUD JORGELINA; RAMOS GUILLERMO; LUQUE ENRIQUE; MUÑOZ-DE-TORO MÓNICA

BIOCELL

Universidad Nacional de Cuyo

Lugar: Mendoza; Año: 2012

0327 9545

exhibits temperature-dependent sex determination (TSD). Male-to-female sex reversal after in ovo estrogen/xenoestrogen exposure was demonstrated (hormonedependent sex determination: HSD). amh, sox9 and sf-1 genes are involved in sex determination/differentiation and steroidogenesis. Ouraimswere:a)toestablishtheexpressionpatternsofamh,sox9 and sf-1 mRNA in the gonad-adrenal-mesonephros (GAM) complexes of neonatalTSD-male and -female, b) to compare gene expressions between TSD-females and HSD-females, c) to evaluateifinovoexposuretoxenoestrogens(E2,bisphenolA-BPA orendosulfan-END)modifyamh,sox9orsf-1mRNAexpressions in neonatal TSD-males. mRNA expressions were quantitatively compared by RT-PCR. We demonstrated a sexually dimorphic patternofamhandsox9,withhigherexpressioninTSD-malethan in -female. sf-1 mRNA did not differ between TSD-males and females. HSD-females exhibited a higher expression of sox9 than TSD-females. In neonatal TSD-males, increased mRNA expression of sex-determining genes was observed after in ovo exposure to END. E2 decreased the sox9 but increased the sf-1 mRNA expression. Changes induced by BPA were evident although no significant. Our results provide new insights into the potential mechanisms that lead to the gonadal histo-functional alterations observed in caimans exposed to contaminated environments.Durando M, Cocito L, Rodríguez HA, Varayoud J, Ramos JG, LuqueEH,Muñoz-de-ToroM. Lab. de Endocrinología y Tumores Hormonodependientes. Fac. Bioq. y Cs. Biol. Univ. Nac. del Litoral. Argentina. E-mail: mdurando@fbcb.unl.edu.ar