congresos y reuniones científicas
Papain-Poly (vinyl sulfonate) Complex Formation as the First Stage in Bioseparation
BRAIA, MAURICIO; PORFIRI, MA. CECILIA; FERRERO, MAXIMILIANO; ROCHA, MARÍA V.; TUBIO, GISELA; ROMANINI, DIANA
Congreso; 16th International Conference on BioPartitioning and Purification, BPP; 2011
This work reports investigation aiming at verifying the existence of specific interactions and insoluble complex formation between papain from Carica papaya and poly (vinyl sulfonate) using spectroscopy techniques. The papain poly(vinyl sulfonate) complex was insoluble at pH lower than 6, with a polymer/protein molar ratio of 1/279. The presence of low ionic strength (0.25M) can easily redissolve the complex. This suggests that electrostatic forces are involved in the interaction between the protein and the polymer. Papain activity in the presence of poly (vinyl sulfonate) is higher than in its absence and the variation of the activity through time is lower in the presence of the polymer. In this way, poly (vinyl sulfonate) protects the enzyme from itself degradation and makes it more stable. The recovery obtained after PAP precipitation with PVS from latex was high (86.98 %) which prove that the methodology is suitable for concentrating the enzyme or as a first extractive step in the purification designs. However, the purification factor obtained was low (2.08). We are working to find the conditions that can improve the purification factor.