Protocols on Micropropagation of Medicinal Plants.

SANSBERRO P.; KOTIK D.; TARRAGÓ J.; LUNA C,; MROGINSKI L.

Tissue Culture of Achyrocline spp. (Asteraceae).

Jahangirnagar University, Bangladesh

Lugar: Bangladesh; Año: 2007;

Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides and Achyrocline alata. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30 g·L-1) containing different combinations of 6-benzyladenine (BA) and a-naphtalenacetic acid (NAA) under 116 mmol·m-2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. Organogenesis was markedly influenced by the type of explants in A. alata. After 30 d of culture in MS plus NAA at 0.5 mM and BA at 5 mM , the regeneration rate from cotyledons, hypocotyls, and leaf explants was 12±12,  50±14.4, and 94±2.8 %, respectively. In A. satureioides the regeneration was similar for every tested explant and varied between 64 and 83 %. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate in vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised in vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.