INVESTIGADORES
PETRUCCELLI Silvana
congresos y reuniones científicas
Título:
Immunoproteomic studies for epitope mapping of cross-reactive allergens of cow´s milk and soy as an approach to mucosal vaccine design
Autor/es:
TREJO, SEBASTIAN; CANDREVA ANGELA; FERRER, M; BRONSOMS, S; DOCENA, GUILLERMO; PETRUCCELLI, SILVANA
Lugar:
Bilbao
Reunión:
Workshop; The 2nd Metabolomics & Proteomics Workshop ? Technologies and Applications;; 2012
Institución organizadora:
CIC bioGUNE (Center for Cooperative Research in Biosciences
Resumen:
Soy-based formulas are frequently used as a dairy substitute in paediatric patients with cow´s milk allergy (CMA). However, clinical intolerance has been observed in some patients and it might be due to the cross-reactivity between these allergenic systems. Bovine caseins, and 7S and 11S soy glycinins have been described as cross-reactive allergens and we propose to explore the immunomodulation of CMA through the use of cross-reactive components by immunotherapy. Novel therapeutic strategies beyond the strict avoidance of allergen exposure are urgently needed and immunotherapy is an attractive optionfor specific and inexpensive corrective treatment for many diseases. A key point for this therapeutic approach isthe identification of the antigenic component that can be use to induce an immune component that may either block the antigen (neutralization), or induce specific regulatory T cells (tolerance). Using monoclonal antibodies (mAb) specific to bovine caseins, we have investigated the antigenic properties of CM and soy proteins. In order to identify the epitopes recognized by these mAbs, purified caseins were enzymatically digested. The resulting peptides were separated by RP-HPLC and analyzed by dot blot using three casein-specific mAbs, and finally they were identified by MALDI-TOF MS. In parallel, antibodies were immobilized onto magnetic beads and afterwards incubated with the peptide mixture previously obtained by proteolysis. The MALDI-TOF analysis was performed to compare bound and unbound peptide profiles. Using different proteolytic enzymes we have shown that casein-specific mAbs recognize at least three peptides (two on αS1-casein and one on Κ-casein). By means of bioinformatic tools we have found that these immunogenic epitopes are also expressed in different soy proteins previously described as allergens. In conclusion, we have detected cross-reactive soy and milk epitopes that could be use as vaccine candidate to develop tolerance in patients with food allergy