REUNION ANUAL DE BIOCIENCIAS Y SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA SAIC

MARTINEZ MARIGNAC VERONICA; OERTLIN GLORIA; GHILINI, DANIEL; FLEISCHMAN, ERIKA; RICHARD, SILVINA

Encuentro; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Toll-like receptors (TLRs) may be involved in the initial failure of viralclearance and in the subsequent development of fatal clinical man-ifestations of severe COVID-19,essentially ARDS (acute respiratorydistress syndrome).Here we characterized the expression of TLR 3,4, and 7 in nasopharyngeal total RNA samples from 150 individualspositive for Covid19 by Neokit SA and 150 non detectable ambula-tory patients; we compared with the symptomatology developed.Weanalyzed 4 cohorts: Viral genome detected patients with severe tohigh symptomatology(n=107);Viral genome detected patients with-out or low symptomatology(n=43);Viral genome non detected pa-tients with severe to high symptomatology(n=109); andViral genomenon detected patients without or low symptomatology(n=41).Weshowed significant differences of expression for TLR4 between de-tected and non-detected patients with severe or non-severe symp-toms (Non Paired T?test pValue=0.04; and pValue=0.04).TLR3showed significant differences between non detected and detectedpatients with severe symptoms(Non Paired T-test; pValue=0.003).The expression of TLR7 was not significant different between co-horts however when compared by Spearman?s Correlation Coeffi-cient there was a significant positive correlation between severityof symptoms in those patients without covid19 infection. The samesignificant positive correlation was observed for TLR4 and severesymptoms in no covid19 patients by Spearman?s and Point-BiserialCorrelation.On average all detected patients expressed these genesin a same way as non-detected patients with severe symptoms,been TLR4 the significant higher expressed gene in all patients withsevere symptoms;we confirmed that the immune response can beassociated with harm to the host due to persistent inflammation andtissue destruction; we confirmed TLR4 gene expression involve-ment in the pathogenesis of COVID‐19 as well as other respiratorydiseases;we suggest that effort must be done to focus therapies onTLR4 expression in inflammatory respiratory diseases.