Daily variations of blood biochemical parameters in Passer domesticus.

PADRONES MN; ARIAS RJ; RONCHI GD; CID, FD; CAVIEDES-VIDAL E; CHEDIACK, JG

San Juan

Congreso; Segunda Reunión Conjunta de Sociedades de Biología de la República Argentina.; 2011

Sociedad Argentina de Biologia - Sociedad de Biologia de Cuyo - Sociedad de Biologia de Cordoba

DAILY VARIATIONS OF BLOOD BIOCHEMICAL PARAMETERS IN Passer domesticus. EFFECT OF FASTING.Padrones N, Arias R, Ronchi GD, Cid FD, Caviedes-Vidal E, Chediack JG. Laboratório de Biología Integrativa. IMIBIO-SL. CONICET. UNSL. San Luis. ArgentinaIn mammals and birds starvation is characterized into three sequential phases (F1, F2 and F3) that are defined by physiological changes, such as rate of mass loss or changes in biochemical parameters. In small birds these changes occur in few hours, and are not known how these parameters change during day. Our objective was to determine the daily variations of biochemical parameters and the effect of fasting. To test our goal, for daily variations experiment twenty Passer domesticus were acclimated to laboratory conditions with water and food ad libitum and then four groups were established (8:00, 12:00, 16:00 and 20:00 hs). For fasting experiment, five groups were established (Control, F1:4hs, F2:8 y 12hs, F3:32hs). We measured hematocrits (Hto) and plasma concentrations of triglycerides (TG), uric acid (UA), total protein (TP) and albumin (Al). We found daily variations (without fasting) in TG, UA and Al, but not in TP and Hto. TG and UA show a peak at 16:00 h, and Al increase during the day until 20:00hs. During fasting, profiles of TG, TP and Al plasma levels and hematocrit, are similar during F1 and F2, but change at F3 (32 hs of fasting). UA change during all phases of fasting, showing a peak at 32hs of fasting. The last phase of fasting produces drastic variations in metabolites profiles in plasma, mainly in uric acid and hematocrit.