Diclofenac effects on the health status of Zenaida auriculatta.

ROSALES, G; FILIPPA VP; PEREZ, E; CHEDIACK, J. G.; CID, F. D.

San Juan

Congreso; XLI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2023

SBCuyo

Diclofenac (DFC) is a contaminant ofemerging concern of great current significance, widely used in our country asan analgesic in human and veterinary medicine. It has been observed that thisenvironmental pollutant can affect kidney and liver function in certain vertebrates.The toxicity of diclofenac to birds is related to the accumulation of uric acidin the plasma, which can lead to kidney damage and failure. Different birdspecies have varying sensitivities to the drug. However, there is a lack ofknowledge about the effect of DFC in passerines birds and native birds ofArgentina. Thus, the main objective of this work was to elucidate the DFCeffects on the health of Eared Dove (Zenaida auriculata) through theevaluation of biochemical parameters, body weight and daily water intake. Four groups (n=7)were exposed during 10 days to 0.005 mg/kg (G1), 0.25 mg/kg (G2) and 2.5 mg/kg(G3) DFC concentration administered by intermittent gavage each day at the sametime, and one control group that were administered only with water without DFC.Before (ti) and after (tf) exposure, body weight was measured and bloodextraction were performed at the same time (9:00 am), to avoid disturbing bycircadian or daily variation. Water intake was measured daily during the tendays of exposure. Concentration of different biochemical parameters weredetermined using a CM200 Automatic Biochemistry Analyzer Wiener Lab, likealbumin, alkaline phosphatase, cholinesterase, glucose, GOT, GPT, totalproteins, triglycerides, urea, and uric acid. Statistical analysis forbiochemical parameters was performed using RM-ANOVA, and one-way ANOVA for bodyweight and water intake results (Tukey post-hoc test, p < 0.05). Wefound a significant decrease in the albumin and total proteins concentrations (p > 0.05) between G3group and control group. Besides, these concentrations were decreased betweenG3 tf and G3 ti (p > 0.05). The other parameters analyzedremained constant through all treatments. These results suggest that theadministration of DFC at a concentration of 2.5 mg/kg might be affecting liverfunction. Consistent with reports in other species, subtle hepatocellulardisruption could occur during chronic liver damage, resulting in normal GPTvalues but decreased albumin and other plasma proteins. However, furtherhistological studies are necessary to support this observation.