A preliminary evaluation of atrazine effect on the disaccharidases enzymes of eared dove (Zenaida auriculata).

MANZANOS N; BACH N; CID F.; CHEDIACK, JG

San Luis

Congreso; XXIX Reunion Anual de la Sociedad de Biología de Cuyo; 2021

Sociedad de Biología de Cuyo

Theaim of this project is to investigate the adequacy of functions, related todigestive processes, in birds exposed to environmental contaminants. The digestivetract has a high capacity to adapt to different conditions, both internalsignals and external agents, in this sense it has been observed that certainenvironmental pollutants can affect the enzymatic activity of the digestivesystem in some vertebrates. However, this effect is poorly studied in birds.Considering that atrazine is a widely used pesticide in our country, andworldwide, and that negative effects on the physiology of some organisms havealso been described, we propose as the general objective of this study toelucidate the long-term Atrazine effects on digestive enzymes of Eared Dove (Zenaida auriculata). To achieve our goal, weestablished three independents groups of birds (n=6 each group), two groupswere exposed during 15 days to the 25 mg/kg and 250 mg/kg atrazine concentrationin corn oil administered by intermittent gavage, and one control group thatwere administered only with corn oil without atrazine. After exposure, bodyweight measure, blood extraction and subsequent removal of the intestine,stomach, liver and pancreas were performed at the same time (8:00 am), to avoiddisturb by circadian/daily variation. The intestinal enzymatic activity ofsucrose and maltase was determined and statistical analysis performed (RM-ANOVA,Tukey post-hoc test, p<0.05). No difference between groups is observed in themasses of the digestive system organs. The pattern found of intestinal enzymesactivities were similar as those reported for other bird species. Althoughatrazine has been shown to have pathological effects in some species of birds, thereis a lack of knowledge about effect on the digestive physiology. Our resultsshowed a non-significant inhibition of these enzymes in doves exposed toAtrazine. Supported by CyT-UNSL PROICO 02-0820 and FONCYT PICT-201?0595