IN VIVO AND IN VITRO ATRAZINE EVALUATION ON DIGESTIVE ENZYMES OF EARED DOVE (Zenaida auriculata)

MANZANOS N; BACH N; CID F.; CHEDIACK, JG

Mendoza

Congreso; XL Reunion Anual de a Sociedad de Biologia de Cuyo; 2022

Sociedad de Biologia de Cuyo

It has been observed that certain environmentalpollutants can affect the enzymatic activity of the digestive system in somevertebrates. However, this effect is poorly studied in birds. Atrazine is awidely used herbicide in our country, and worldwide, and negative effects onthe physiology of some organisms have been described. In these sense, althoughatrazine has been shown to produce some adverse effects in birds, there is alack of knowledge about the effect on digestive physiology. Thus, the main objectiveof this work was to elucidate the Atrazine effects on digestive enzymes (intestinaland pancreatic) of Eared Dove, “in vivo”model, and the direct effect in brush border membrane vesicles (BBMV), in vitro model. To achieve our goal, for in vivo experiment, we established threeindependent groups of birds (n=6 each group), two groups were exposed during 15days to the 25 mg/kg and 250 mg/kg atrazine concentration. After exposure, body weight measure, blood extractionand removal of the intestine, stomach, liver and pancreas were performed at thesame time (8:00 am), to avoid disturb by circadian/daily variation. BBMV was obtained after intestinal lumenscraping using a polyethylene glycol (PEG) technique, then, BBVM were exposedto Atrazine concentrations of 5 µM; 2.5 µM; 1 µM and 0.5 µM, taking intoaccount two controls, one with buffer and the other with ethanol, sinceatrazine is solubilized at 0.4% in ethanol. The intestinal enzymatic activity of sucrase, maltaseand aminopeptidase was determined in both in vivo and in vitromodels and the enzymatic activity of the pancreatic enzymes trypsin andchymotrypsin was determined in vivomodel. Statistical analysis performed was RM-ANOVA to comparethe proximal, medial, and distal portions of the in-vivo model and forpancreatic enzymes and the in-vitro model, one-way ANOVA, Tukey'spost-hoc test, p<0 .05. We did not found any differences in the masses ofthe organs of the digestive system. We did not found effect of Atrazine in theintestinal digestive enzymes nor pancreatic enzymes. We found a classicalpattern of intestinal enzyme activity reported for other bird species. Ourresults showed a non-significant direct inhibition of intestinal enzymesstudied in BBMV exposed to different concentrations of Atrazine.  In conclusion, the doses assayed of atrazinedo not affect the intestinal and pancreatic digestive enzymes in doves. Supported by CyT-UNSL PROICO 02-0820 and FONCYTPICT-201–0595.