Kisspeptins and their receptors in the brain-pituitary-gonadal axis of Odonthestes bonariensis : Their relationship with gametogenesis along the reproductive cycle.

BOHÓRQUEZ, M. OSWALDO TOVAR; MECHALY, ALEJANDRO S.; ELISIO, MARIANO; CHALDE, TOMÁS; CANOSA, LUIS F.; MIRANDA, LEANDRO A.; SOMOZA, GUSTAVO M.

GENERAL AND COMPARATIVE ENDOCRINOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2017

0016-6480

n vertebrates, the reproduction is controlled by the brain-pituitary-gonadal(BPG) axis and kisspeptin has emerged as a key player of this axis. In thisstudy, we analyzed changes in the expression levels of kiss1, kiss2, and theirreceptors, kissr2 and kissr3 during gametogenesis in the BPG axis of feralOdontesthes bonariensis. In females, levels of brain kiss1 showed an increaseat final maturation (Fm), while kiss2 levels were shown to be high at primarygrowth (Pg) stage, with no differences in the expression of their receptors. Inthe pituitary, kiss1 and kiss2 peaked at the cortical alveoli (Ca) stage, andkissr3 at initial vitellogenesis. In parallel, there was an increase of kiss1, kissr2 and kissr3 in the ovary during the Ca stage and both receptors again at Fm stage. In males, the four genes were highly expressed in the brain at thearrested (A) stage. In the pituitary, kiss2 peaked at spermatogonial (SG) andspermatocytary (SC) stages; while kissr3 reached a peak at the spermiogenicstage (SP). In testes, kiss1 and kiss2 significantly increased during the SG andSC stages; meanwhile, kissr2 increased at SG and SC, whereas kissr3 levelswere significantly high at SC and SP stages. Taken together these resultsshowed that the kisspeptin system in pejerrey is expressed in the three levelsof the BPG axis with different expression profiles during the gonadal cycle.These findings pointed that kisspeptins have different roles in gametogenesisin this species.