INVESTIGADORES
AWRUCH Cynthia Andrea
artículos
Título:
Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks
Autor/es:
AWRUCH C. A.; SIMPFENDORFER, C.; PANKHURST, N. W
Revista:
MARINE AND FRESHWATER RESEARCH
Editorial:
CSIRO PUBLISHING
Referencias:
Año: 2011 vol. 62 p. 694 - 699
ISSN:
1323-1650
Resumen:
Measurement of lactate is becoming a common procedure in assessing the physiological effects of capture
stress in sharks, although the necessity to measure the concentrations in the laboratory limits the ability for field
assessments. Portable lactate analysers offer an alternative, but await validation against laboratory assays for sharks. The
present study assessed the reliability of a portable Lactate Pro analyser for measuring whole-blood lactate in the school
shark, Galeorhinus galeus, in the field. Laboratory determination of whole-blood and plasma lactate obtained by
spectrophotometry was highly correlated with field determinations. Because shark lactate concentration can exceed the
upper detection limit of the portable analysers, which were designed for mammalian use, a method for dealing with values
greater than the maximum detection limit was evaluated. Whole-blood diluted by 50% with acidified saline solution, tap
water and distilled water gave measured values of 55, 56 and 52%, respectively, of the original values, allowing accurate
estimation of concentrations exceeding the upper detection limit of the analyser. These findings indicated that the Lactate
Pro can be used to rapidly and reliably measure lactate for sharks in the field.
spectrophotometry was highly correlated with field determinations. Because shark lactate concentration can exceed the
upper detection limit of the portable analysers, which were designed for mammalian use, a method for dealing with values
greater than the maximum detection limit was evaluated. Whole-blood diluted by 50% with acidified saline solution, tap
water and distilled water gave measured values of 55, 56 and 52%, respectively, of the original values, allowing accurate
estimation of concentrations exceeding the upper detection limit of the analyser. These findings indicated that the Lactate
Pro can be used to rapidly and reliably measure lactate for sharks in the field.
spectrophotometry was highly correlated with field determinations. Because shark lactate concentration can exceed the
upper detection limit of the portable analysers, which were designed for mammalian use, a method for dealing with values
greater than the maximum detection limit was evaluated. Whole-blood diluted by 50% with acidified saline solution, tap
water and distilled water gave measured values of 55, 56 and 52%, respectively, of the original values, allowing accurate
estimation of concentrations exceeding the upper detection limit of the analyser. These findings indicated that the Lactate
Pro can be used to rapidly and reliably measure lactate for sharks in the field.
Galeorhinus galeus, in the field. Laboratory determination of whole-blood and plasma lactate obtained by
spectrophotometry was highly correlated with field determinations. Because shark lactate concentration can exceed the
upper detection limit of the portable analysers, which were designed for mammalian use, a method for dealing with values
greater than the maximum detection limit was evaluated. Whole-blood diluted by 50% with acidified saline solution, tap
water and distilled water gave measured values of 55, 56 and 52%, respectively, of the original values, allowing accurate
estimation of concentrations exceeding the upper detection limit of the analyser. These findings indicated that the Lactate
Pro can be used to rapidly and reliably measure lactate for sharks in the field.