EFFECT OF PHYTODERIVATIVES ON THE GROWTH OF HOMOLOGOUS BENEFICIAL VAGINAL LACTOBACILLI (BVL) STRAINS AND THEIR COMPATIBILITY FOR THE DESIGN OF PHYTOBIOTICS FOR THE VAGINAL TRACT HEALTH

ANTONELLA MARCHESI; JESSICA ALEJANDRA SILVA; ARISTIMUÑO FICOSECO MARIA CECILIA; BIRGITT WIESE

World Journal of Pharmacy and Pharmaceutical Sciences

Faculty of Pharmacy

Lugar: Bulgaria; Año: 2020 vol. 9

2278 4357

Lactobacilliare the predominant bacteria in the vaginal tract, and while they are present,can prevent infectious situations, being used as probiotics for the health ofthe tract. Phyto derivatives are used historically to prevent or maintain thehealth of the tract, and is possible to combine both bioactives for novelvaginal formulas. The aim of this work was to study the effect ofphytoderivatives (individual or combined extracts) on the growth of homologous vaginallactic acid bacteria and their compatibility, to go further in the design of a combinedpharmabiotic formula for the prevention or treatment of urogenital tractinfections. Methods: the effect of phytocompounds approved by Pharcopoeias wasevaluated on the growth of beneficial lactic acid bacteria previously isolated fromthe vaginal tract. Statistical methods, as CART, were applied to determine thestimulatory or inhibitory effect of the vegetal sources on bacterial growth byapplying different methodology. Compatibility between phyto and lactobacilliwas determined, and the phenolic compounds contents quantified, to define theoptimal conditions for the formula design. Results: The BVL strains showinghighest growth values with most of the phytoderivatives under study were L. gasseri CRL 1361, L. ga. CRL 1509 and L. ga. CRL 1263, and L.jensenii CRL 1333 and L. jen. CRL 1317, indicating the behavior isstrain-dependent, and vegetable-compound-affected. The most adequatephytoderivatives were: Carica, Centella, Plantago, Uva ursi*, Zarzaparrila*, Colade caballo, Echinaceae, Ortiga and Palo Azul. Conclusions: The BVL strainsshowing optimal resistance to the phytocompounds and compatibility with them wereL. gasseri CRL 1320, 1307, 1509, L. salivarius CRL 1296 and L. rhamnosus CRL 1332 combined with Uva ursi*,Zarzaparrilla* and Echinaceae that will be used for the design of optimal phytobioticformulas.