EFFECT OF PGE2 ON THE FUNCTIONS OF NEUTROPHILS DURING HUMAN TUBERCULOSIS

CANDELA MARTIN; JOAQUIN PELLEGRINI; MARIA PAULA MORELLI; NANCY TATEOSIAN; AMIANO NICOLAS; CIALLELLA LORENA; DOMINGO J. PALMERO; GARCÍA VERÓNICA

Buenos Aires

Congreso; Reunión conjunta SAIC-SAI-SAFIS 2020- Virtual; 2020

Neutrophils have been associated with tuberculosis (TB) protection but also withexcessive inflammatory burden. Previously we showed that PGE2 decreased CD11bexpression in Mtb -Ag stimulated neutrophils from healthy donors (HD) . Here weinvestigated the potential role of PGE2 on human neutrophils´ response during activeTB. We evaluated the expression of immunoreceptors (PD-L1, PD-L2), thegeneration of reactive oxygen species (ROS) and the induction of autophagy.Neutrophils were obtained from heparinized peripheral blood from HD andtuberculosis patients (TB) and cultured (2x10 6 cells/ml) with a Mycobacteriumtuberculosis lysate ( Mtb -Ag, 10μg/ml) ± PGE2 (2μM). ROS production andPD-L1/PD-L2 surface expression were determined by flow cytometry. Confocalmicroscopy and flow cytometry were used to evaluate autophagy levels. P-values