DETERMINATION OF THE FREQUENCY OF MYELOID-DERIVED SUPPRESSOR CELLS DURING HUMANACTIVE TUBERCULOSIS

CASTELLO FLORENCIA; MARIA PAULA MORELLI; JOAQUIN PELLEGRINI; AMIANO NICOLAS; CASCO, NICOLÁS; A LORENA CIALLELA,; DOMINGO J. PALMERO; GARCÍA V.E; NANCY TATEOSIAN

Congreso; Reunión conjunta SAIC-SAI-SAFIS 2018; 2018

Determination of the Frequency of Myeloid-derived Suppressor Cells during human Active tuberculosis.Tuberculosis (TB), together with HIV infection, is the leading cause of death from an infectious disease worldwide. In fact, Mycobacterium tuberculosis (Mtb) causes almost 10 million of new cases and 1.5 million of deaths per year. Myeloid-derived suppressor cells (MDSCs) display an immunosuppressive function during several pathological conditions such as cancer and hepatitis. MDSC-mediated suppression of host immunity during chronic inflammation is crucial for immune regulation and tolerance to limit immunopathology. Furthermore, the unfavorable effects of MDSCs are evident in tumor biology where they accumulate and suppress cytokine cytokines Th1 responses. An effective host immune response against Mtb largely depends on the generation of Th1 cytokines such as IFN-. Then, the aim of this work was to study the expression and role of MDSCs during human active pulmonary tuberculosis infection. Thus, we investigated the frequency of granulocytic MDSCs (g-MDSCs) and monocytic MDSCs (m-MDSCs)) in peripheral blood mononuclear cells from TB patients classified as high (HR-TB) or low (LR-TB) responders on the basis of their immunological response to Mtb-antigen. Using flow cytometry, we observed that both g-MDSCs (CD14- CD11b+ CD15+) and m-MDSCs (CD14+ CD11b+ HLA-DR-/low) showed significantly higher levels (P