CENEXA 05419
CENTRO DE ENDOCRINOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
artículos
Título:
Increasing DNA Isolation Performance from Blood Cells Using a Modified Commercial Low-Cost Reagent
Autor/es:
ABBA MC; MENCUCCI MV; GAGLIARDINO JJ; KRAEMER MN; LACUNZA E
Revista:
CLINICAL LABORATORY.
Editorial:
CLIN LAB PUBL
Referencias:
Lugar: Bensheim; Año: 2019 vol. 65 p. 2179 - 2183
ISSN:
1433-6510
Resumen:
Background: Genomic studies facilitate comprehension of the pathophysiology, diagnosis, and treatment of chronic diseases. Such studies require sufficient and good quality DNA isolated from a large number of blood samples. This study attempts to obtain a high-quality genomic DNA isolated from a large number of blood samples using a simple and cheap method.Methods: The EasyPure® Genomic DNA Kit (Transgen Biotech) was modified to increase the amount of DNA recovery: a few steps and two additional column elutions were added to the original manufacturer´s procedure.Results: The amount of DNA isolated from frozen blood samples increased by an average of 56%. Its 260/280 ratio and electrophoretic mobility properties make it suitable for genomic studies.Conclusions: A relatively low-cost commercial column and a simple modification of the manufacturer´s protocol, provided a simple and cheap procedure to isolate high-quality DNA from a large number of blood samples suitable for genomic studies.
