INVESTIGADORES
CEBALLOS Santiago Guillermo
artículos
Título:
A rapid approach for sex assignment by RAD-seq using a reference genome
Autor/es:
PERALTA, DIEGO M.; TÚNEZ, JUAN I.; RODRÍGUEZ CRUZ, ULISES E.; CEBALLOS, SANTIAGO G.
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Año: 2024 vol. 19
ISSN:
1932-6203
Resumen:
Sexidentificationis a commonobjectivein molecularecology.Whilemanyvertebratesdis-playsexualdimorphism,determiningthesexcanbechallengingin certainsituations,suchasspecieslackingclearsex-relatedphenotypiccharacteristicsor in studiesusingnon-inva-sivemethods.In thesecases,DNAanalysesserveasvaluabletoolsnotonlyforsexdeter-minationbutalsoforvalidatingsexassignmentbasedonphenotypictraits.In thisstudy,wedevelopeda bioinformaticframeworkforsexassignmentusinggenomicdataobtainedthroughGBS,andhavinganavailablecloselyrelatedgenomeassembledat thechromo-somelevel.Ourmethodconsistsof twoadhocindexesthatrelyonthedifferentpropertiesof themammalianheteromorphicsexchromosomes.Forthispurpose,wemappedRAD-seqlocito a referencegenomeandthenobtainedmissingnessandcoveragedepthvaluesfortheautosomesandX andY chromosomesof eachindividual.Ourmethodologysuccess-fullydeterminedthesexof 165fursealsthathadbeenphenotypicallysexedin a previousstudyand40sealionssampledin a non-invasiveway.Additionally,weevaluatedtheaccu-racyof eachindexin sequenceswithvaryingaveragecoveragedepths,withIndexY prov-inggreaterreliabilityandrobustnessin assigningsexto individualswithlow-depthcoverage.Webelievethattheapproachpresentedherecanbeextendedto anyanimaltaxawithknownheteromorphicXY/ZWsexchromosomesystemsandthatit cantoleratevariousqualitiesof GBSsequencingdata.
