Identification of Claudin-2 and SGLt1 in Galea musteloides

BASTÓN, J. I.; CHEDIACK, J.G.; ENRIQUE CAVIEDES VIDAL

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2005

IDENTIFICATION OF CLAUDIN-2 AND SGLTl IN Galea musteloides Bastón JI, Chediack JG, Caviedes-Vidal E. Lab Biol., Univ Nac San Luis, 5700 Sun Luis. E-mail: jibaston@yalzoo.com.ar Several protein complexes govern the absorption of water-soluble solutes from the intestinal lumen into the organism. Claudin-2, a transmembrane protein of the tight junction complex has been reported as a major component involved in the permeability of the paracellular pathway. On the other hand, SGLTl, the Na+/Glucose co-transporter located at the brush border membrane of the small intestine, is a relevant component of the glucose uptake through the transcellular pathway. The aim of our study was to identify claudin-2 and SGLTl in a very precocial rodent, Galea musteloides. Animals used in this study were randomly chosen from our own in-captivity colony at UNSL. SDS-PAGE and Western blot analysis were performed in duodenal and jejunal brush border membranes isolated from the small intestine. We also assayed Wistar rat tissues to validate the specificity of the antibodies. Immunoblotting was developed by an ECL chemiluminescence system. Characteristic bands for claudin-2 and SGLTl were obtained. This technique will provide an useful tool for to study the development of intestinal absorption function in G. musteloides.