Estradiol on coeliac ganglion and ovaric incubation modifies in differential manner the relation between the cell-extracellular matrix and α, β-actin in ovary on dioestus II

BRONZI D,; FOSCOLO M; RODRIGUEZ D; SOSA ZY; VEGA OROZCO A; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXXII Reunión Científica Anual. San Luis; 2014

ESTRADIOL ON COELIAC GANGLION AND OVARIC INCUBATION MODIFIES IN DIFFERENTIAL MANNER THE RELATION BETWEEN THE CELL-EXTRACELLULAR MATRIX AND α, β-ACTIN IN OVARY ON DIOESTUS II. Bronzi CD, Foscolo M, Rodriguez D, Sosa ZY, Vega Orozco A, Casais M. Lab. Biol. Reprod. (LABIR). FQBF-UNSL, IMIBIO-SL, CONICET. San Luis. Argentina. E-mail: cdbronzi@gmail.com Cell-extracellular matrix (ECM) interactions are important in the ovaric apoptotic mechanisms. These interactions are mediated by integrins, which participates in the junction between ECM to cytoskeleton in the cell. The aims were to evaluate whether estradiol (E2) on coeliac ganglion (CG) of the ex vivo coeliac ganglion- superior ovarian nerve-ovary system and on ovary alone (OV) is capable of modify in ovary on DII: 1- GnRH and E2 release; 2) the interaction of cytoskeletal proteins (α, β-actin) with the extracellular matrix. The ex vivo CG-SON-O system and OV were removed and placed in one cuvette containing Krebs Ringer solution at pH 7.4 without (control groups) and with E2 added to ganglion in the system or the OV (experimental groups). The ovarian liquid was extracted at 120 min and GnRH and E2 were determinated by RIA. The presence of α and β-actin in ovary were determinated by immunofluorescence. ANOVA 1 followed by Bonferroni test with a statistical significance of **p