Effect of nitric oxide on ovarian estradiol release and its synthesis enzyme expression, in the first proestrus in the rat

MORALES LD; DELSOUC MB; VALLCANERAS S; DELGADO SM; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXXII Reunión Científica Anual. San Luis; 2014

EFFECT OF NITRIC OXIDE ON OVARIAN ESTRADIOL RELEASE AND ITS SYNTHESIS ENZYME EXPRESSION, IN THE FIRST PROESTRUS IN THE RAT Morales LD, Delsouc MB, Vallcaneras S, Delgado SM, Casais M. Lab Biol Reprod (LABIR). FQByF-UNSL, IMIBIO-SL CONICET, San Luis. E-mail: lauradanimorales@hotmail.com The nitric oxide (NO) is a gaseous neurotransmitter involved in steroidogenesis and follicular development. In the first proestrus (PE), the stage where is evident follicular maturation which lead to the first ovulation, the ovarian steroid hormone with major participation is estradiol (E2). Taking into account this information, the aims of this work, in the first PE, were to study the effects of NO synthase (NOS) inhibitors and a NO donor on: 1) the release of E2 in ovary without neural influence (Ov), 2) ovarian gene expression of P540 aromatase (P450arom, E2 synthesis enzyme). The Ov was incubated in Krebs-Ringer buffer at 37°C (control group). Inhibitors of NOS: aminoguanidine 400 µM (AG) and L-nitroarginine methyl ester 100 µM (L-NAME), selective and non-selective of inducible NOS, respectively; and NO donor: sodium nitroprusside 100 µM (SNP), were added in Ov. E2 was determined by RIA at 120 min and 180 min. One-way ANOVA and Tukey test were used (p