Effect of TNFRP55 deficiency on the pg synthesis at the end of pregnanacy

DE LA VEGA M; RAGUSA JA; ANZULOVICH AC; CASAIS M

Congreso; Sociedad de Biología de Cuyo XXXII Reunión Científica Anual. San Luis; 2014

EFFECT OF TNFRP55 DEFICIENCY ON THE PG SYNTHESIS AT THE END OF PREGNANACY De la Vega M, Ragusa JA, Anzulovich AC, Casais M. Lab Biol Reprod (LABIR)-FQByF-UNSL, IMIBIO-SL-CONICET - San Luis. E-mail: magalidlv0@gmail.com.ar An adequate rhythm of progesterone (Pg) is crucial for determining the physiological duration of the estrous cycle and for achieving a successful pregnancy. Tumor necrosis factor is a pleiotropic cytokine and TNF receptor p55 (TNFRp55) mediates most of the TNF effects. We previously showed that Pg levels and its synthetic enzyme (3β-HSD) exhibit a circadian rhythm in the ovary in diestrus. In this work we investigate the consequences of the lack of TNFRp55 in the Pg metabolism at the end of pregnancy, in C57BL/6 wild type (WT) and TNFRp55-/- (KO) mice. Animals were maintained on a 12-h light: 12-h dark cycle, at 24±2°C, with irradiated food and water available ad-libitum. Five days before the experiment mice were kept under constant darkness conditions. Ovaries were isolated every 6 h (24-48 h before delivery) during a 24h period. Pg and 3β-HSD protein levels were determined by RIA and Western blot, respectively. Similar to that we previously observed in diestrus, Pg levels are circadian in the ovary of WT mice (p