A simple method for taking photographs of histological sections without using neither photographic camera nor microscope

CARMANCHAHI, PABLO; ALDANA MARCOS, HERNÁN; FERRARI, CARINA; AFFANNI, JORGE.

BIOCELL

Secretariat

Año: 1998 vol. 22 p. 207 - 210

0327-9545

A simple photographic method for histological sections is described. This is a contact method which does not require the use photographic camera It is very similar to the contact print method used routinely in photographic laboratories. It is performed by placing the slides, with the histological section, coverslip downwards over the emulsion of the negative film. Then, they are illuminated with a common photographic enlarger during a brief period of time. Thus, a negative contact print of the section is obtained. This technique yields photographs of  high quality focus, sharply contrasted with great detail. These results may also be obtained with low-contrast stained sections. This method is most suitable for photography of large or medium-sized histological sections (macros). This is often the case of neuroanatomical and immunohistochemical studies. Our method, being simple and quick, is recommended for routine use in laboratories.