INVESTIGADORES
CANOSA luis fabian
congresos y reuniones científicas
Título:
DETERMINATION OF THE NUTRITIONAL REQUIREMENTS OF PEJERREY (Odontesthes bonariensis): ANALYSIS OF THE SOMATOTROPIC AXIS AS THE MAIN BIOMARKER OF GROWTH IN TELEOSTS.
Autor/es:
GOMEZ-REQUENI P; SOMOZA GM; CANOSA LF
Lugar:
Chascomus
Reunión:
Conferencia; 2da Conferencia Latinoamericana sobre Cultivo de Peces Nativos.; 2009
Resumen:
The pejerrey (Odontesthes bonariensis) is a fish species
with a huge potential for the aquaculture industry but, nowadays, growth rates
and survival in cultured conditions are not successful. In order to establish
the nutritional requirements and optimal feed intake of pejerrey, tissue gene
expression of somatotropic axis-related hormones (GH, GHRs, IGF-I and IGF-II)
as well as myogenin and myostatin as muscle growth markers, and the screening
of the proteomic profile of liver and skeletal muscle of fish affected by
different nutritional conditions will be examined. Different diets varying on protein
and lipid content (P/L) will be evaluated. In addition, growth promotion of different
feeding rates such as a) ad
libitum, b) 1% of body weight
(BW) and c) 2% BW will be examined.
Expression levels of target genes mentioned above will be quantified by means
of real time-PCR in order to estimate the growth promotion effect of the diets
and feeding rates. In order to develop those tools, muscle growth markers
myogenein and myostatin from pejerrey have been partially cloned. These
sequences as well as pejerrey IGF-I and GH sequences were used establish a quantitative
determination of its expression levels.