Dietary protein and lipid levels affect growth and expression of growth-related genes in pejerrey larvae (Odontesthes bonariensis)

BERTUCCI JI; TOVAR O; HERDMAN JE; CANOSA LF; UNNIAPPAN S

London, Ontario

Congreso; Canadian Society of Zoologiest Annual Meeting; 2016

Canadian Society of Zoologiest

Pejerreyis an argentinian fish appreciated for its commercial importance. This workaimed to determine if food protein and lipid affect biometric parameters, and expressionof genes implicated in somatic growth, fatty acid metabolism and food intakeregulation. Larvae were fed with diets containing different proportion ofprotein (P) and lipid (L) (12%L-40%P (control), 20%L-40%P, 12%L-50%P or20%L-50%P) during 45 days. Total number, biomass and standard length weremeasured throughout the experiment, and gene expression was quantified at theend. Larvae fed with low L diets show the highest increase in weight andstandard length, although no differences were observed in the condition factor,survival rate and food intake. Feeding on 20%L-40%P diet led to an increase inhead gh expression and body ghr-II and NUCB2/nesfatin-1, while produced a decrease in head NUCB2/nesfatin-1 and Δ6-desaturase. Larvae fed on a 12%L-50%Pdiet showed increased head igf-II andNUCB2/nesfatin-1 but decreased gh and Δ6-desaturase levels. Expression of head igf-II and body ghr-Iwere downregulated by 20%L-50%P diet, while Δ6-desaturasewas upregulated. This work show that changes in dietary protein and lipidcontent affect pejerrey growth and body weight and modulate the expression ofgenes involved in somatic growth and food intake behavior.