Determinación de carbamazepina en suero humano asistida por quimiometría y comparación con métodos de referencia

MS CÁMARA, C MASTANDREA, HC GOICOECHEA Y Y HERRERA DÍAZ

Santiago de Cuba

Congreso; 18 Conferencia de Química de Cuba; 2005

Sociedad Cubana de Quimica

In the present report, carbamazepine is determined on serum samples of real patients by a procedure completely assisted by chemometric tools. First, a response surface methodology based on a mixture design was applied in order to select the best conditions for the extraction step. Finally, partial least squares multivariate calibration (PLS-1) was applied to second derivative UV spectra, eliminating a shift baseline effect originated in the extraction procedure. The performance assessment included: a) a three level precision study, b) a recovery study analysing spiked samples, and c) a method comparison with high performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA) applied on real patient samples. The obtained results show the potentiality of the presently studied methodology for the monitoring of patients treated with this anticonvulsivant. Keywords: Carbamazepine; serum samples; Chemometrics; Partial least squares regression; systematic optimization