INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Surface exploration of a room-temperature ionic liquid-chitin composite film decorated with electrochemically deposited PdFeNi trimetallic alloy nanoparticles by pattern recognition: An elegant approach to developing a novel biotin biosensor
Autor/es:
GHOLIVAND M; JALALVAND A; GOICOECHEA HC; PAIMARD G; SKOV T
Revista:
TALANTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 131 p. 249 - 258
ISSN:
0039-9140
Resumen:
In this study, a novel biosensing system for the determination of biotin (BTN) based on electrodeposition
of palladium-iron-nickel (PdFeNi) trimetallic alloy nanoparticles (NPs) onto a glassy carbon electrode
(GCE) modified with a room-temperature ionic liquid (RTIL)-chitin (Ch) composite film (PdFeNi/ChRTIL/
GCE) is established. NPs have a wide range of applications in science and technology and their sizes are
often measured using transmission electron microscopy (TEM) or X-ray diffraction. Here, we used a
pattern recognition method (digital image processing, DIP) for measuring particle size distributions
(PSDs) from scanning electron microscopic (SEM) images in the presence of an uneven background.
Different depositions were performed by varying the number of cyclic potential scans (N) during
electroreduction step. It was observed that the physicochemical properties of the deposits were
correlated to the performance of the PdFeNi/ChRTIL/GCE with respect to BTN assay. The best results
were obtained for eight electrodeposition cyclic scans, where small-sized particles (19.5476.27 nm)
with high density (682 particles mm2
) were obtained. Under optimized conditions, a linear range from
2.0 to 44.0 109 mol L1 and a limit of detection (LOD) of 0.6 109 mol L1 were obtained. The
PdFeNi/ChRTIL nanocomposite showed excellent compatibility, enhanced electron transfer kinetics, large
electroactive surface area, and was highly sensitive, selective, and stable toward BTN determination.
Finally, the PdFeNi/ChRTIL/GCE was satisfactorily applied to the determination of BTN in infant milk
powder, liver, and egg yolk samples.